blue alkaline phosphatase alp substrate kit (Vector Laboratories)
Structured Review

Blue Alkaline Phosphatase Alp Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1591 article reviews
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1) Product Images from "Maternal Exercise Rescues Fetal Akinesia‐Impaired Joint and Bone Development"
Article Title: Maternal Exercise Rescues Fetal Akinesia‐Impaired Joint and Bone Development
Journal: The FASEB Journal
doi: 10.1096/fj.202503192R
Figure Legend Snippet: Maternal exercise alters growth plate morphometry and bone neovascularization. (A) Fluorescent staining for collagen type 10 alpha 1 chain (Col10a1) in the proximal hypertrophic zone (HZ) of embryonic day 17.5 (E17.5) humeri. Scale bar = 500 μm. (B) HZ length. (C) Fluorescent staining for alkaline phosphatase (ALP) activity in E17.5 humeri. Scale bar = 250 μm. (D) Primary ossification center (POC) ALP activity and (E) bone collar (BC) ALP activity. (F) Fluorescent staining for blood vessels (endomucin) in E17.5 humeri. Scale bar = 250 μm. (G) Medullary vessel area, (H) Medullary vessel density, (I) BC vessel area, (J) BC vessel density, (K) metaphyseal vessel area, and (L) metaphyseal vessel density. Fetuses from the same litter are marked by the same color datapoint. * p < 0.05 using a Student's t ‐test.
Techniques Used: Staining, Activity Assay
Related Articles
Staining:Article Title: Maternal Exercise Rescues Fetal Akinesia‐Impaired Joint and Bone Development Article Snippet: For all protocols, samples were mounted using Fluoromount‐GT (Thermo Fisher Scientific), imaged using the ZEISS AxioScan.Z1 Slide Scanner (ZEISS, Oberkochen, Germany), and analyzed using ImageJ/Fiji. .. Alkaline phosphatase (ALP) staining was conducted using the Vector Article Title: Multisite studies for validation and improvement of a highly efficient culture assay for detection of undifferentiated human pluripotent stem cells intermingled in cell therapy products. Article Snippet: Each 1 mL of cell suspension using E8F +Y medium or AK03N+Y medium was respectively seeded on 10-cm culture dishes coated with laminin-521 (BioLamina AB) [23] or 10- cm culture dishes containing iMatrix-511 solution (0.25 mg/cm2) [25]. .. The day after the seeding, media were replaced with each respective medium without Y-27632, and after 5 or 6 days of incubation at 37°C in 5% CO2, the dishes were fixed with 4% paraformaldehyde phosphate buffer solution and stained using a Vector Article Title: International evaluation study of a highly efficient culture assay for detection of residual human pluripotent stem cells in cell therapies. Article Snippet: Four wells were prepared for each concentration, and wells in which only 1 × 106 iCell cardiomyocytes were seeded (nonspiked sample) were prepared for each experiment (n = 1 or 2). .. Three days after seeding, the media were replaced with Essential 8 Flex medium, and after a total of 7 days of incubation at 37◦C in 5% CO2, the wells were fixed and stained using a Vector Article Title: Maternal exercise rescues fetal akinesia-impaired joint and bone development Article Snippet: For all protocols, samples were mounted using Fluoromount-GT (Thermo Fisher Scientific), imaged using the ZEISS AxioScan.Z1 Slide Scanner (ZEISS, Oberkochen, Germany), and analyzed using ImageJ/Fiji. .. Alkaline phosphatase (ALP) staining was conducted using the Vector Incubation:Article Title: Multisite studies for validation and improvement of a highly efficient culture assay for detection of undifferentiated human pluripotent stem cells intermingled in cell therapy products. Article Snippet: Each 1 mL of cell suspension using E8F +Y medium or AK03N+Y medium was respectively seeded on 10-cm culture dishes coated with laminin-521 (BioLamina AB) [23] or 10- cm culture dishes containing iMatrix-511 solution (0.25 mg/cm2) [25]. .. The day after the seeding, media were replaced with each respective medium without Y-27632, and after 5 or 6 days of incubation at 37°C in 5% CO2, the dishes were fixed with 4% paraformaldehyde phosphate buffer solution and stained using a Vector Article Title: International evaluation study of a highly efficient culture assay for detection of residual human pluripotent stem cells in cell therapies. Article Snippet: Four wells were prepared for each concentration, and wells in which only 1 × 106 iCell cardiomyocytes were seeded (nonspiked sample) were prepared for each experiment (n = 1 or 2). .. Three days after seeding, the media were replaced with Essential 8 Flex medium, and after a total of 7 days of incubation at 37◦C in 5% CO2, the wells were fixed and stained using a Vector |
